Assembling a unique three-copper center of thiocyanate dehydrogenase in vitro

Key Points

• Enhanced catalytic activity was observed with the engineered copper center, demonstrating improved function.
• The study highlights the importance of copper ions in enzymatic reactions related to thiocyanate.
• In vitro techniques were employed to precisely assemble the unique three-copper structure of the enzyme.
• These findings may enable further exploration of thiocyanate dehydrogenase's biochemical roles in various systems.