Photoclickable HaloTag Ligands for Spatiotemporal Multiplexed Protein Labeling on Living Cells

Key Points

• The research aims to develop a photoclickable labeling system for enhanced protein tracking in living cells.
• Developed photoclickable HaloTag ligands
• Applied the platform in living cell environments
• Tested the precision of protein labeling
• Achieved selective marking of proteins in living cells
• Demonstrated spatiotemporal control over fluorescence labeling
• Enhanced multiplexing capabilities for tracking multiple proteins