Nup107 depletion in Drosophila larvae caused a complete arrest in pupariation due to disrupted EcR signaling, highlighting its critical role in metamorphosis regulation.
Nup107 acts as a crucial epistatic regulator of Torso-mediated activation of EcR signaling during metamorphic transition in Drosophila.
Absolute Event Rate: 0% vs 0%
Nuclear pore complexes (NPCs), composed of nucleoporins (Nups), affect nucleocytoplasmic transport, thus influencing cell division and gene regulation. Nup107 subcomplex members have been studied in housekeeping functions, diseases, and developmental disorders. We report a unique regulatory function for Nup107 in metamorphic transition during Drosophila development. RNA interference (RNAi)-mediated Nup107 -depleted larvae were arrested in the third-instar larval stage with no signs of pupariation. This lack of pupariation is primarily due to inhibited nuclear translocation and transcriptional activation by EcR. We demonstrate the involvement of Nup107 in the transcription of the Halloween genes, modulating ecdysone biosynthesis and the EcR pathway activation. The regulation of EcR-mediated metamorphosis by the receptor tyrosine kinase, torso , is well documented. Accordingly, overexpression of the torso and MAP-kinase pathway activator, ras V12 , in the Nup107 depletion background rescues the phenotypes, implying that Nup107 is an epistatic regulator of Torso-mediated activation of EcR signaling during metamorphosis.
Kawadkar et al. (Tue,) reported a other. Nup107 depletion in Drosophila larvae caused a complete arrest in pupariation due to disrupted EcR signaling, highlighting its critical role in metamorphosis regulation.