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March 13, 2026Kirkuk University Journal For Agricultural Sciences0 citationsOpen Access

In Vitro Evaluation of the Efficacy of Plant Extracts, Biological Agents, and Chemical Pesticides in Controlling Xanthomonas spp Bacteria Isolated from Tomato Plants in Erbil, Kurdistan Region, Iraq

LMLina Musa MahmodSalahaddin University-Erbil KFKamalaldeen M. FatahSalahaddin University-Erbil

Key Points

This research aims to evaluate the efficacy of various plant extracts, biological agents, and chemical pesticides against Xanthomonas spp. affecting tomato plants in Erbil.
Aqueous extracts of medicinal plants were prepared and tested against isolated Xanthomonas spp.
Molecular identification of bacterial isolates was conducted to confirm species identities.
Effectiveness of Bacillus subtilis and Trichoderma harzianum as bioagents was assessed.
Commercial pesticides were tested for their antibacterial activity against the bacterial isolates.
Clove extract exhibited strong antibacterial activity against X. euvesicatoria and X. campestris.
Bacillus subtilis showed strong antagonistic effects against both bacterial species.
Himex pesticide demonstrated substantial antibacterial activity (47.37%–54.51%).
Aryal Super was ineffective against the pathogens.
Ginger extract showed moderate inhibition of X. campestris, while other plant extracts were ineffective.

Abstract

Tomato (Solanum lycopersicum L.), coming in the second level in production and consumption over the entire world after cereals. It is an important source of essential vitamins, minerals and antioxidants Tomato provides an estimated 80% of daily intake of lycopene, folate, ascorbic acid, ﬂavonoids, and potassium in the western diet. China, India, USA, Turkey, and Egypt are the major top producers of tomato over the entire world. According to the Ministry of Agriculture and Water Resources / Kurdistan Regional Government, total tomato production in 2021 was 248546 tons distributed over the four governorates. Xanthomonas spp. is the most significant tomato pathogen, causing losses ranging from 10% to 60% of the crop yield. Aqueous extracts of four medicinal plants, Maidenhair Fern, Turmeric, Clove, and Ginger alongside bioagents (Bacillus subtilis, Trichoderma harzianum) and commercial pesticides (Himex and Aryal Super) were evaluated for their antibacterial activity against Xanthomonas spp. isolated from infected tomato plants. Isolation, pathogenicity testing, and molecular identification confirmed the presence of X. euvesicatoria, and X. campestris. Clove extract shows strong antibacterial activity against both isolates, whereas, Ginger was moderately active against X. campestris. The bioagent, B. subtilis strongly active against the isolates, T. harzianum was ineffective. The commercial pesticide Himex was strongly effective, while Aryal Super was ineffective. Clove extract shows high inhibition rate on both X. euvesicatoria and X. campestris at 10 mg/mL. Ginger extract only moderately inhibits X. campestris at 100 mg/mL, while turmeric and maidenhair fern were not effective. B. subtilis strongly antagonistic against both bacterial species, whereas T. harzianum was ineffective. Among the chemical treatments, Himex demonstrated substantial antibacterial activity (47.37%–54.51%), while Aryal Super showed no inhibition.The findings suggest that clove extract and Bacillus subtilis are promising eco-friendly alternatives to conventional pesticides for managing bacterial leaf spot in tomato cultivation.

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