Interfacial Redox Transformation and Phytochemical Capping Drive the Formation and Protective Bioefficacy of Phyllanthus acidus-Derived Selenium Nanoparticles against Aeromonas hydrophila Infection in Zebrafish

Key Points

• The research aims to explore the creation and protective capability of selenium nanoparticles from Phyllanthus acidus against Aeromonas hydrophila in zebrafish.
• Selenium nanoparticles were synthesized using Phyllanthus acidus fruit extract as a reductant.
• Characterization of nanoparticles was performed through various profiling techniques including LC–MS/MS-QTOF.
• Functional assays were conducted in a zebrafish model to evaluate protective effects against bacterial infection.
• The synthesized selenium nanoparticles demonstrated significant protective bioefficacy against Aeromonas hydrophila in zebrafish.
• Profiling revealed the presence of gallic acid among other bioactive phytochemicals involved in nanoparticle formation.
• The study supports the potential of Phyllanthus acidus-derived nanoparticles for application in aquaculture.