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March 18, 2026Lishizhen medicine and materia medica research0 citations

Research on the effect of Fructus Lycii Ruthenic and Herba Cistanches on senile osteoporosis in aged rats via regulation of the PI3K/Akt signaling pathway

Key Points

This research explores the impact of Fructus Lycii and Herba Cistanches on senile osteoporosis in aged rats through modulation of the PI3K/Akt signaling pathway.
48 SD rats were divided into several groups including a control and treatment groups.
Micro-CT assessed bone microstructure changes in the treated rats.
HE staining analyzed femur pathology, while alkaline phosphatase staining measured osteoblast activity.
TRAP staining assessed osteoclast activity, and ELISA measured serum TNF-α, BALP, and TRACP-5b levels.
Western blot analyzed the expression of PI3K/Akt pathway proteins in femur tissue.
Compared to the control group, aged rats showed significant degradation in bone microstructure and decreased BMD/PV/TV, Tb.N, and Tb.Th values (P<0.05).
The treatment group exhibited improvements in these parameters and reduced pathological damage (P<0.05).
Rats treated with 740 Y-P showed trends opposite to the treatment group, suggesting its counteractive effects.

Abstract

目的探讨黑果枸杞+肉苁蓉通过调控磷脂酰肌醇3-激酶（PI3K）/丝氨酸-苏氨酸激酶（Akt）信号通路对老年性骨质疏松症（SOP）大鼠的影响。方法 48只SD大鼠随机分为空白组、衰老组、VE组（0.018 g·kg-1）、PI3K激活剂组（740 Y-P组，10 mg·kg-1）、黑果枸杞+肉苁蓉组（1 440 mg·kg-1）、黑果枸杞+肉苁蓉+740 Y-P组。Micro-CT检测大鼠骨微结构变化；HE染色观察股骨病理形态、碱性磷酸酶钙-钴染色检测大鼠股骨中成骨细胞活性、抗酒石酸酸性磷酸酶（TRAP）染色检测大鼠股骨组织中破骨细胞活性；ELISA法检测血清肿瘤坏死因子-α（TNF-α）、骨碱性磷酸酶（BALP）、抗酒石酸酸性磷酸酶5b（TRACP-5b）含量；Western blot检测股骨组织PI3K/Akt通路蛋白表达。结果与空白组比较，衰老组大鼠股骨微结构明显破坏，BMD、BV/TV、Tb.N、Tb.Th显著下降（PPPPP 结论黑果枸杞+肉苁蓉可通过调控PI3K/Akt通路改善SOP。

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FENG et al. (2026) studied this question.

synapsesocial.com/papers/69ba43764e9516ffd37a4cbb https://doi.org/10.70976/j.1008-0805.szgygy-2026-0406

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March 18, 2026Lishizhen medicine and materia medica research0 citations

Research on the effect of Fructus Lycii Ruthenic and Herba Cistanches on senile osteoporosis in aged rats via regulation of the PI3K/Akt signaling pathway

PFPeng FENG XXXiaomin Xu YYYing Yi

Key Points

This research explores the impact of Fructus Lycii and Herba Cistanches on senile osteoporosis in aged rats through modulation of the PI3K/Akt signaling pathway.
48 SD rats were divided into several groups including a control and treatment groups.
Micro-CT assessed bone microstructure changes in the treated rats.
HE staining analyzed femur pathology, while alkaline phosphatase staining measured osteoblast activity.
TRAP staining assessed osteoclast activity, and ELISA measured serum TNF-α, BALP, and TRACP-5b levels.
Western blot analyzed the expression of PI3K/Akt pathway proteins in femur tissue.
Compared to the control group, aged rats showed significant degradation in bone microstructure and decreased BMD/PV/TV, Tb.N, and Tb.Th values (P<0.05).
The treatment group exhibited improvements in these parameters and reduced pathological damage (P<0.05).
Rats treated with 740 Y-P showed trends opposite to the treatment group, suggesting its counteractive effects.

Abstract

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Cite This Study

FENG et al. (2026) studied this question.

synapsesocial.com/papers/69ba43764e9516ffd37a4cbb https://doi.org/10.70976/j.1008-0805.szgygy-2026-0406

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