What question did this study set out to answer?

The aim is to explore how vitamin A affects milk fat synthesis in H₂O₂-injured bovine mammary cells.

March 26, 2026Open Access

Vitamin A Alleviates the Reduction in Milk Fat Synthesis through the TrxR/PPARγ Pathway in H₂O₂-Injured BMECs .1

Key Points

The aim is to explore how vitamin A affects milk fat synthesis in H₂O₂-injured bovine mammary cells.
Silenced TrxR using small interfering RNA (siRNA).
Allocated third-passage bovine mammary epithelial cells (BMECs) into six treatment groups.
Evaluated the role of vitamin A in the TrxR signaling pathway.
Inhibited PPAR activity using GW9662.
Vitamin A alleviated the reduction of milk fat synthesis caused by H₂O₂.
Silencing TrxR showed a significant effect on milk fat synthesis.
Blocking PPAR activity negatively impacted the beneficial effects of vitamin A.

Abstract

This study aimed to investigate the effect and mechanisms by which vitamin A (VA) alleviates the reduction in milk fat synthesis by silencing TrxR and blocking the PPAR in bovine mammary gland epithelium cells (BMECs) injured by H 2 O 2 .TrxR was silenced by using small interfering RNA (siRNA) to detect the role of TrxR in milk fat synthesis.The third-passage BMECs were allocated to 6 treatments including CON, H 2 O 2 , VA+H 2 O 2 , siTrxR, siTrxR + VA, and siTrxR + VA+H 2 O 2 , to evaluate the mechanism of VA alleviating the decline of milk fat synthesis by TrxR signaling pathway.Subsequently, GW9662 (GW) was used to inhibit PPAR activity.Cells were randomly assigned to 6 treatments including CON,

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Cite This Study

Zhao et al. (Thu,) studied this question.

synapsesocial.com/papers/69c4cc69fdc3bde4489179ca https://doi.org/https://doi.org/10.1017/anr.2026.10034

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