Meibum HCunsat decreases with age and is lower with dry eye, Sjögren's syndrome, and Parkinson's disease cohorts compared with a cohort without dry eye. This decrease in HCunsat is expected to contribute to lipid aggregation, decreased lipid spreading, and meibomian gland outflow. ¹H-NMR was effective for quantifying major meibum lipids.
Machane et al. (Mon,) studied this question.