What question did this study set out to answer?

The aim is to find methods for improving peptide-protein conjugation while maintaining disulfide bonds.

April 1, 2026Chemical Science0 citationsOpen Access

A (poly)Pro tip for preserving native disulfide connectivity during thiol-maleimide bioconjugation of disulfide-rich peptides

LALylia AzzougCentre National de la Recherche Scientifique ANAna NovakCentre National de la Recherche Scientifique HMHervé MeudalCentre National de la Recherche Scientifique

Key Points

The aim is to find methods for improving peptide-protein conjugation while maintaining disulfide bonds.
Investigated methods for conjugating disulfide-rich peptides to protein carriers.
Analyzed the impact of disulfide connectivity on immunogenicity.
Compared conventional and novel bioconjugation techniques.
Achieved improved retention of disulfide connectivity during bioconjugation.
Enhanced immune response observed in models with preserved disulfide bonds.
Demonstrated that antibody production was significantly increased compared to traditional methods.

Abstract

Generation of specific antibodies against peptides by immunization requires their covalent conjugation to protein carriers to override their inherently weak immunogenicity. The vast majority of bioconjugation approaches to achieve peptide-protein...

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