A robust and reliable reverse-phase high-performance liquid chromatography (RP-HPLC) method was developed and validated for the estimation of Itraconazole (ITZ) in pharmaceutical dosage forms. The method showed excellent linearity (R2 = 0.999) in the concentration range of 1–25µg/mL, high accuracy with recovery between 96.8 and 99.3%, and precision with %RSD less than 2.0%. The limits of detection (LOD) and quantification (LOQ) were 2.12µg/mL and 6.43µg/mL, respectively, confirming high sensitivity. Beyond conventional validation, the method was evaluated for sustainability using greenness and whiteness assessment tools (AGREE = 0.71, AGREEprep = 0.72, RAPI = 72.5, ComplexGAPI = 85, WAC = 72.5). The integrated evaluation confirmed that the analytical method is robust with moderate greenness, aligning with Green Analytical Chemistry (GAC) principles and partially contributing to the UN Sustainable Development Goals (SDGs).
Arghode et al. (Thu,) studied this question.