Abstract 797: Integrating hematoxylin & eosin histology with multiplexed imaging mass cytometry for spatial proteomic profiling of antibody-drug conjugate targeted biomarkers.

Key Points

• This work aims to enhance histopathological insights through the integration of H&E staining with imaging mass cytometry to identify ADC-related biomarkers.
• Developed a workflow combining H&E-stained sections with IMC antibody panels targeting ADC biomarkers.
• Selected regions of interest based on histological features for IMC acquisition and analysis.
• Performed computational analysis for cell phenotyping and spatial mapping of biomarkers.
• Achieved high registration accuracy when aligning H&E images with IMC segmentation maps across various tissue types.
• Demonstrated quantitative capability to detect and enumerate cells expressing ADC targets like HER2 and EGFR.
• Uncovered spatial patterns of biomarker distribution that correlate with histopathological features.