Duplex-specific nuclease-assisted isothermal signal amplification coupled with HPLC-UV for simultaneous detection of multiple microRNAs

Key Points

• The aim is to develop a highly sensitive method for the simultaneous detection of multiple microRNAs as potential disease biomarkers.
• Developed a duplex-specific nuclease-assisted isothermal amplification technique
• Coupled with HPLC-UV for analyzing amplified signals
• Tested in a controlled laboratory setting with relevant biological samples
• Successfully detected multiple microRNAs simultaneously
• Showed increased sensitivity compared to traditional methods
• Identified potential biomarkers for various diseases through amplification techniques