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September 11, 1972Physical Review Letters1,762 citations

Thermodynamic Fluctuations in a Reacting System—Measurement by Fluorescence Correlation Spectroscopy

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DMDouglas MagdeEEElliot ElsonWWW. W. Webb

Key Points

  • To measure temporal correlations of thermodynamic concentration fluctuations in a reactive equilibrium system to simultaneously determine chemical rate constants and diffusion coefficients.
  • Applied fluorescence correlation spectroscopy to detect spontaneous fluorescence fluctuations of reaction products at chemical equilibrium.
  • Analyzed the equilibrium binding interaction between ethidium bromide and DNA.
  • Extracted chemical rate constants and diffusion coefficients directly from the temporal correlation of equilibrium fluctuations.
  • Demonstrated the experimental observation of coupling between molecular diffusion and chemical reaction kinetics.

Abstract

The temporal correlations of thermodynamic concentration fluctuations have been measured in a chemically reactive system at equilibrium by observing fluctuations of the fluorescence of a reaction product. The experiment yields the chemical rate constants and diffusion coefficients and shows the coupling among them. Data are reported for binding of ethidium bromide to DNA.

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Cite This Study

Magde et al. (1972) studied this question.

synapsesocial.com/papers/69d82e388c03fbaff8bedf0chttps://doi.org/10.1103/physrevlett.29.705
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