Study on the Effect of Critical High Temperatures in the VIGS-TRV System on the Silencing of the Tobacco NtPPO Gene

Key Points

• The aim is to clarify the effect of critical high temperatures on the silencing of the NtPPO gene using the VIGS-TRV system.
• Investigated NtPPO7 and NtPPO8 gene expression at temperatures of 25℃, 40℃, 43℃, and 46℃.
• Measured changes in TRV concentration corresponding to temperature variations.
• Analyzed gene silencing efficiency at different temperatures.
• At 40℃, gene silencing efficiency was lower compared to 25℃.
• Silencing effects disappeared at 43℃ and 46℃.
• Gene silencing efficiency decreased over time in plants treated at 25℃ after initial exposure to 40℃.