Nanoassembly-enabled aqueous solid-phase peptide synthesis (ASPPS): a practical DMF-free approach based on the Fmoc strategy

Key Points

• The aim is to present a new method for peptide synthesis using nanoassembly that avoids harmful solvents.
• Introduced a nanoassembly-based approach for peptide synthesis.
• Utilized Fmoc-amino acids forming non-molecularly dissolved domains in water.
• Conducted synthesis under aqueous coupling conditions.
• Demonstrated enhanced reactivity for peptide synthesis.
• Achieved rapid and practical outcomes without using harmful DMF.