Multiplexed Fluorescent Polymeric Microstructures Sequentially Encoded via Two-Photon Click Tetrazole-based Chemistry.

Key Points

• This work aims to develop a method for spatially controlled functionalization of polymeric microstructures using two-photon reactions.
• Developed a direct laser writing method for microstructures.
• Applied two-photon triggered reactions to achieve functionalization.
• Utilized tetrazole-alkene cycloaddition for fluorescence activation.
• Achieved successful spatial control in polymeric microstructure functionalization.
• Demonstrated fluorescent turn-on capability via the cycloaddition reactions.