Isoform‐specific function underlies differential herbicide resistance of the W574L mutation in peanut AhALS isozymes

Key Points

• The aim is to investigate how different isoforms of peanut ALS affect herbicide resistance due to specific mutations.
• Analyzed the effects of the W574L mutation across different AhALS isozymes.
• Evaluated the functional differences among isoforms.
• Conducted assessments to determine phenotypic outcomes of the same mutation in varying protein structures.
• Identified that the W574L mutation leads to different resistance levels depending on the isozyme.
• Found that AhALS2b is not a suitable target for herbicide editing due to its ineffectiveness.