Combining Rational Design and Computational Tools in Multi-Parameter Enzyme Engineering to Increase the Fitness of a CYP152 Peroxygenase for α-Hydroxylation of Fatty Acids | Synapse
April 10, 2026Open Access
Combining Rational Design and Computational Tools in Multi-Parameter Enzyme Engineering to Increase the Fitness of a CYP152 Peroxygenase for α-Hydroxylation of Fatty Acids
Key Points
The aim is to enhance the fitness of CYP152 peroxygenase for specific fatty acid hydroxylation.
Utilized rational design techniques alongside computational tools for enzyme engineering.
Focused on regioselective and stereoselective hydroxylation of fatty acids.
Applied hydrogen peroxide as the oxidant.
Achieved increased efficiency in transforming fatty acids to α-hydroxy acids.
Demonstrated improved regio- and stereoselectivity in the enzymatic reactions.
Abstract
Enzymes of the CYP152 family have the potential to upgrade fatty acids to α-hydroxy acids by regio-and stereoselective hydroxylation using hydrogen peroxide as the oxidant and forming water as the...