What question did this study set out to answer?

This research aims to explore how TICRR affects lung adenocarcinoma cell growth and its underlying mechanism.

April 10, 2026Open Access

Topoisomerase II binding protein 1-interacting checkpoint and replication regulator promotes lung adenocarcinoma proliferation by regulating myelocytomatosis oncogene signaling pathway

Key Points

This research aims to explore how TICRR affects lung adenocarcinoma cell growth and its underlying mechanism.
Analyzed TICRR expression in lung adenocarcinoma patients using Cancer Genome Atlas database.
Conducted loss- and gain-of-function experiments on LUAD cells to assess TICRR's impact on proliferation.
Performed pathway enrichment analysis and luciferase reporter assays to investigate the mechanism.
TICRR overexpression correlates with poor survival in LUAD patients (P < 0.001).
Increased TICRR levels boosted LUAD cell proliferation, while depleting TICRR reduced it.
TICRR activated the MYC signaling pathway by influencing Bcl-2-associated X protein and Cyclin B1.

Abstract

Objectives: This study aims to investigate the role of topoisomerase II binding protein 1-interacting checkpoint and replication regulator (TICRR) on the proliferation of lung adenocarcinoma (LUAD) and its potential molecular mechanism as well as to provide a new strategy to improve LUAD treatment. Material and Methods: The expression level of TICRR in patients with LUAD was analyzed on the basis of the Cancer Genome Atlas program database, and loss- and gain-of-function experiments were conducted to validate whether TICRR promoted the proliferation of LUAD cells. Then, pathway enrichment analysis and luciferase reporter assays were performed to dissect the potential mechanism. Results: Overexpression of TICRR in patients with LUAD was associated with poor survival ( P < 0.001). In addition, overexpression of TICRR aggravated LUAD cell proliferation, which was ameliorated by TICRR depletion. In addition, TICRR could activate the myelocytomatosis oncogene (MYC) signaling pathway by regulating Bcl-2-associated X protein and Cyclin B1, which are the pivotal effectors of the MYC signaling pathway. Conclusion: TICRR plays a critical role in fostering the progression of LUAD by regulating the MYC signaling pathway.

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