The current study addresses the need for more accessible analytical techniques over complex methodologies. The present work aimed to develop and validate methods using potato tubers across genotypes, storage conditions (4 °C and 15 °C), and tuber anatomical regions, with challenges like starch interference mitigated by testing various clarifying agents and digestion approaches. Among these, acetate salt with warm digestion emerged as an optimal treatment, yielding stable, interference-free optical rotational measurements. The polarimetric method was statistically validated against standard colorimetric methods using linear regression, a correlation matrix, and hypothesis testing. Regression models showed strong linearity (R2 = 0.99 for sucrose and 0.98 for glucose), with slopes approximating unity and minimal intercept biases. Also, nonsignificant differences (p > 0.05) were analyzed between the data sets, confirming the polarimetric method’s efficiency. This work contributes a validated protocol for compositional analysis of root crops and supports broader inclusion of such methods in food composition databases.
Goraya et al. (Thu,) studied this question.