An organo-diselenide functionalized BODIPY probe was synthesized and its structure was confirmed through NMR spectroscopic analysis, including ( 1 H, 13 C and 77 Se) along with mass spectrometric analysis. The reactivity of probe with various Reactive Oxygen Species (ROS) and biologically relevant thiols was systematically investigated. The probe selectively detect superoxide via “ turn-off ” fluorescence response with a detection limit of 9.65 µM. The probe exhibited quick fluorescence quenching response towards superoxide anion without interference from other ROS and biothiols. Further, in addition to sensing capability, the probe was examined for biological activities, and was displayed significant antibacterial and anti-inflammatory properties, highlighting its potential applicability in both chemical sensing and biomedical studies.
Deshmukh et al. (Fri,) studied this question.