What question did this study set out to answer?

To sequence and analyze the genomes of two chromogenic bacteria isolated from environmental sources.

April 22, 2026Microbiology Resource Announcements0 citationsOpen Access

Whole-genome sequencing of two chromogenic bacteria, Exiguobacterium spp. and Pseudomonas chlororaphis , isolated from environmental sources in Northern Colorado

TMTaylor MellingColorado State University CMCarolina MehaffyColorado State University

Key Points

To sequence and analyze the genomes of two chromogenic bacteria isolated from environmental sources.
Utilized Nanopore technology for sequencing
Performed de novo assembly of genomes
Annotated genomes for antibiotic resistance genes
Generated one complete circular genome for Pseudomonas chlororaphis
Produced three circular contigs for Exiguobacterium spp., including two plasmids
Identified various antibiotic resistance genes in both genomes

Abstract

Exiguobacterium spp. (EA-TM-5) and Pseudomonas chlororaphis (PC-TM-6) were sequenced via Nanopore technology. De novo assembly resulted in one circular contig representing the complete genome of P. chlororaphis (PC-TM-6) and three circular contigs for Exiguobacterium spp. (EA-TM-5) representing its complete genome and two plasmids. Annotation revealed various antibiotic resistance genes.

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