Objective: Teeth decay disease is a huge health problem and some reports have inferred caspase recruitment domain family member 8 (CARD8), inflammasome sensor involvement in the disease. Several single nucleotide polymorphisms (SNPs) have been detected in the CARD8 gene. The reference SNP 2043211 (rs2043211) variant is situated in its exon region, leading to the dental caries. The study aimed to determine the impact of the CARD8 (rs2043211SNPs) on the susceptibility of caries presence in patients.Methods: The case-control study included 38 participants aged 20-30 years divided into two groups; the first group (17) was healthy with non-carious teeth and the other group (21) was with active decayed teeth. The clinical examination was done for caries detection then the peripheral blood was taken from all included participants of both groups. The CARD8 gene SNP rs2043211 was amplified and sequenced.Results: Hardy-Weinberg Equilibrium (HWE) analysis of CARDS8 at SNP rs2043211 showed a non-significant (NS) difference in both studied groups. The Adenine Thymine (AT) genotype among the decayed participants was 13 SNPs while in healthy participants 8 SNPs with a non-significant (NS) difference (P=.3). Nevertheless, the odds Ratio (OR) of the AT genotype was 1.83 (0.49-6.7, P=.3) and for genotype TT, it was 1.3 (0.3-5.6, P=.7). It was observed that SNP rs2043211 was associated with tooth decay. In addition, OR for the A allele is 0.6 (0.024-1.5) and 1.65 (0.6-4.15) for the T allele with P=.2 for both alleles.Conclusion: Many factors can play an important role in the initiation and progression of the caries directly or indirectly. The allelic variations in the CARD8 gene are to have been associated with the development of decayed teeth among patients.Keywords: CARD8 gene polymorphism, dental caries, sequencing, teeth decay
Hussein et al. (Thu,) studied this question.