Methods for measuring bone resorption activity using ground pieces of newborn mouse calvaria

Key Points

• This research aims to establish effective methods for measuring bone resorption activity using newborn mouse calvaria.
• Utilized ground pieces of newborn mouse calvaria to assess resorption activity.
• Conducted actual measurement of released [45Ca]-Ca2+ to evaluate calcium release.
• Outlined the number of experimental animals required for accurate measurements.
• Demonstrated a reliable method for quantifying bone resorption activity.
• Presented effective measurement of calcium release from ground bone fragments.