Abstract Purpose To determine the likelihood of obtaining at least one transferable embryo per cycle in patients undergoing IVF with PGT-A + PGT-M for variants in one versus two genes. Methods IVF cycles in patients 18–45 years undergoing PGT-A + PGT-M for variants in one or two genes at a single genetics laboratory were analyzed from 11/2019 to 3/2023. Cycles were stratified by SART age category and disease inheritance pattern. Analyses were performed using median and chi-square tests. Results A total of 4634 IVF cycles were included: 146 cycles (3.15%) using PGT-A + PGT-M for two genes and 4488 cycles (96.85%) for one gene. After adjusting for the number of embryos biopsied, the likelihood of having at least one usable embryo was significantly lower in patients testing for variants in two genes compared to one in patients aged < 35 (75.0% v 82.5%, p = 0.004) and 35–37 (55.0% v 74.4%, p = 0.025) with no difference in other age groups 38–40 (66.7% v 74.4%, p = 0.586) and 41–42 (42.9% v 51.7%, p = 0.113). Conclusions There were significantly lower odds of obtaining a usable embryo when testing for variants in genes in patients aged < 35 and 35–37. No significant difference was seen among patients aged 38–40 or 41–42. These findings provide quantifiable data to counsel patients considering PGT-M testing for variants in two genes.
Rasouli et al. (Fri,) studied this question.