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May 3, 20260 citations

Characterization of a previously uncharacterized MelC2 tyrosinase from Streptomyces griseorubiginosus and development of a whole-cell catalytic system for DOPA-melanin production.

JYJunwen YuanGuangxi University WCWenjie ChengGuangxi University JYJingwen YangGuangxi University

Key Points

This research aims to characterize the MelC2 tyrosinase from Streptomyces griseorubiginosus and develop a whole-cell system for DOPA-melanin production.
Biochemical characterization of MelC2 tyrosinase from S. griseorubiginosus.
Development of a whole-cell catalytic system for DOPA-melanin production.
Optimization of reaction conditions to enhance production yield.
Achieved DOPA-melanin titer of 7.49 g/L under optimized conditions at pH 10.0.
Demonstrated effective use of a whole-cell system for biosynthesis.
First characterization of MelC2 tyrosinase contributes to sustainable bioprocessing.

Abstract

concentration, cell density, and substrate loading. Constant alkaline conditions (pH 10.0) yielded the highest DOPA-melanin production. Under optimized conditions, the engineered whole-cell system achieved a DOPA-melanin titer of 7.49 g/L. This study provides the first biochemical characterization of a previously uncharacterized tyrosinase (MelC2) from S. griseorubiginosus and establishes a whole-cell strategy for sustainable DOPA-melanin biosynthesis.

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