Probiotic potential of Lactobacillus acidophilus TMPC 41127 isolated from traditional cheese in inhibiting Salmonella Typhi biofilms and virulence genes.

: 63.6-79.7 mg/mL). These anti-pathogenic effects were confirmed by observing morphological changes in the pathogen's cells and a reduction in the expression of biofilm and invasion-related genes (csgD, csgA, bcsA, invA, and invC). Additionally, the strain demonstrated other health-promoting properties, including cholesterol reduction (54.62%) and high antioxidant activity. The safety assessments (no hemolytic or DNase activity and no biogenic amine production) and its ability to inhibit the proliferation of cancer cells were also favorable. In conclusion, due to its outstanding probiotic and anti-biofilm properties, the L. acidophilus TMPC 41127 strain is introduced as a promising candidate for food and therapeutic applications.