Vibrio cholerae is a gram‐negative bacterium found in water, particularly in brackish and estuarine environments, and causes cholera—an acute disease transmitted through contaminated food or water. Biofilm formation in V. cholerae enhances its resistance to antimicrobial agents and protection against host immune responses. Natural plant extracts have gained attention for their potential antibiofilm properties against human pathogens such as V. cholerae . This study evaluates the antibacterial and antibiofilm activities of bluestem ( Andropogon spp.) extracts against pathogenic V. cholerae strains using antibacterial assays, biofilm inhibition and eradication assays, and gene expression analysis. Two extracts, denoted as EAV1 and EAV2, primarily composed of two flavonoid C‐glycosides species, demonstrated significant bactericidal effects on V. cholerae . Sub‐MIC levels of the extracts also inhibited the growth of biofilms. Gene expression analysis revealed downregulation of genes encoding biofilm transcriptional regulators, Vibrio polysaccharide, and biofilm matrix proteins upon EAV treatment. Results indicate a potential quorum‐quenching activity by targeting the transcription of biofilm regulators and biofilm matrix genes. These findings suggest that Andropogon spp. extract is a promising antibacterial and antibiofilm agent against V. cholerae , highlighting the potential of plant species with invasive tendencies as sources of antibacterial and antibiofilm compounds against clinically important pathogens.
Caigoy et al. (Thu,) studied this question.