Differential molecular diagnosis test for simultaneous detection of Mpox and Varicella-Zoster viruses during 2023-2024 Mpox outbreak in Rio de Janeiro/Brazil

Key Points

• This research aims to develop a molecular diagnosis test to simultaneously detect Mpox and Varicella-Zoster viruses.
• Developed a differential diagnosis test.
• Conducted testing during the 2023-2024 Mpox outbreak in Rio de Janeiro.
• Analyzed simultaneous detection capabilities for both viruses.
• Successfully detected both Mpox and Varicella-Zoster viruses in outbreak samples.
• Demonstrated test efficacy in real-world outbreak conditions.