Rapid and convenient electrochemical hemoglobin detection in mouse feces employing a DNA aptamer to evaluate the severity of colitis in a mouse model

Key Points

• The research aims to develop a fast method for measuring hemoglobin in mouse feces to assess colitis severity.
• Electrochemical detection technique using a DNA aptamer for hemoglobin quantification.
• Sample collection involved mouse feces with a 20-minute assay duration.
• Cross-reactivity of the aptamer was tested with human hemoglobin.
• Hemoglobin was detected in mouse feces within 20 minutes.
• The method demonstrated high sensitivity in quantifying hemoglobin levels.
• A correlation was observed between hemoglobin levels and colitis severity in the mouse model.