Prior infection with recombinant TTSuV1 induced seroconversion and lymphopenia but did not significantly exacerbate swine influenza virus-associated clinical signs or pulmonary lesions in piglets.
A snatch-farrowed piglet model with recombinant TTSuV1 provides a controlled system for investigating TTV-host interactions and coinfections.
experimental systems. To address these limitations, we investigated the infectivity of a recombinant swine TTV Torque teno sus virus 1 (TTSuV1) generated with a reverse genetics system in a snatch-farrowed piglet model. Additionally, we evaluated the impact of TTSuV1 coinfection on the pathogenesis and replication dynamics of swine influenza virus (SIV). Infection of piglets with the rescued TTSuV1 induced seroconversion and lymphopenia. Early infection was associated with reduced lymphocyte proliferative responses to mitogens and diminished or absent recall responses to viral antigens. TTSuV1 antigen was detected within immune cell populations by flow cytometry. Contrary to expectations, prior TTSuV1 infection did not significantly exacerbate SIV-associated clinical signs or pulmonary lesions. The use of a snatch-farrowed piglet model in combination with recombinant TTSuV1 derived from reverse genetics provides a controlled system for investigating TTV-host interactions. This approach represents a valuable tool for advancing mechanistic studies of TTV biology and its role in modulating coinfections.
Islam et al. (Thu,) conducted a other in Torque teno sus virus 1 (TTSuV1) and swine influenza virus (SIV) coinfection. Recombinant TTSuV1 infection was evaluated on Infectivity, pathogenesis, and replication dynamics of SIV. Prior infection with recombinant TTSuV1 induced seroconversion and lymphopenia but did not significantly exacerbate swine influenza virus-associated clinical signs or pulmonary lesions in piglets.