A multiplex PCR-based sequencing method for the diagnosis and differentiation of echinococcosis using plasma samples, a proof-of-concept study

Key Points

• This research aims to develop and validate a multiplex PCR-based sequencing method for the diagnosis of echinococcosis using plasma samples.
• Developed a multiplex PCR protocol for echinococcosis detection in plasma.
• Tested the method on plasma samples to assess its diagnostic accuracy.
• Demonstrated successful amplification of echinococcosis DNA from plasma samples.
• Indicated potential for improved differentiation between echinococcosis types based on sequencing results.