The authors declare no conflicts of interest. The variant data for this study have been deposited in the European Variation Archive (EVA) at EMBL-EBI under accession number PRJEB80029 (https: //www. ebi. ac. uk/eva/? eva-study=PRJEB80029). Figure S1: Close-up images of affected calf 1 (A) and affected calf 2 (B), showing pink irises and constricted pupils, together with an albino coat phenotype. Figure S2: Region of homozygosity (ROH) analysis, where coloured lines represent runs of homozygosity in each animal. A shared ROH of > 10 Mbps and encompassing the TYR locus (AC₀00186. 1: 6340841–6473276, indicated by a black arrow) was present in both affected animals. This ROH is absent in the Angus control animal and in all three parents (the two dams not shown as no ROH was detected on chromosome 29). Please note: The publisher is not responsible for the content or functionality of any supporting information supplied by the authors. Any queries (other than missing content) should be directed to the corresponding author for the article.
Eager et al. (Fri,) studied this question.