The increasing demand for functional beverages derived from medicinal mushrooms necessitates robust standardization frameworks to ensure product quality, safety, and efficacy. This study presents the development and validation of a Product Internal Standard for Tamogi filter tea formulated from Pleurotus citrinopileatus and synergistic medicinal ingredients, including Cordyceps militaris, Trametes versicolor, Ganoderma spp. and Glycyrrhiza glabra. A multi-dimensional quality control approach integrating sensory, physicochemical, microbiological, and bioactive compound analyses was applied. Key parameters, including moisture content, ash values, and β-glucan concentration, were quantified. Analytical techniques, including UV-Vis spectrophotometry and standardized microbiological assays, were applied to evaluate product quality and safety according to national regulatory frameworks. Heavy metal contamination (Pb, Cd, and As) was determined via ICP-MS. Results demonstrated that the developed product meets stringent quality criteria (moisture 20 mg/mL, absence of pathogenic microorganisms, and heavy metals within permissible limits). Notably, the high β-glucan content supports potential immunomodulatory functionality. The proposed TCCS provides a scalable and scientifically grounded model for the standardization and commercialization of mushroom-based functional teas, contributing to value chain development and sustainable utilization of medicinal fungi.
Phương et al. (Fri,) studied this question.