A CRISPR activation screen identifies CH25H as a restriction factor against influenza viruses by targeting accessible cholesterol

Key Points

• The study aims to identify potential targets in the host that restrict the replication of influenza viruses.
• Utilized CRISPR activation screening to identify restriction factors against influenza viruses.
• Targeted cholesterol metabolism to assess its effects on viral replication.
• Identified CH25H as a key restriction factor against influenza virus replication.
• Demonstrated that targeting 25HC increases therapeutic potential against influenza viruses.