What does this research mean for the field?

A newly developed naphthalimide probe enables selective imaging of lysosomal hydrogen peroxide (H2O2) and reactive oxygen species (ROS) in living cells by forming hydroxylamine without undergoing overoxidation. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

The objective is to develop a method for precise monitoring of hydrogen peroxide distribution in living cells without causing overoxidation.

May 16, 2026RSC Advances0 citationsOpen Access

Selective lysosomal H 2 O 2 –ROS imaging with a naphthalimide probe forming hydroxylamine without overoxidation

RFRicardo Flores-CruzUniversidad Nacional Autónoma de México NRNitzya Ruiz-RobledoUniversidad Nacional Autónoma de México ARAdriana Romo‐PérezUniversidad Nacional Autónoma de México

Key Points

The objective is to develop a method for precise monitoring of hydrogen peroxide distribution in living cells without causing overoxidation.
Developed a naphthalimide probe for selective imaging of hydrogen peroxide in lysosomes.
Evaluated the probe's performance in living cells for dynamic monitoring.
Compartmentalized distribution of hydrogen peroxide was analyzed using advanced imaging techniques.
The naphthalimide probe selectively senses hydrogen peroxide in lysosomes.
Imaging demonstrated precise localization with minimal overoxidation.
Observed dynamic changes in hydrogen peroxide levels under oxidative stress conditions.

Abstract

Hydrogen peroxide (H 2 O 2 ) plays a central role in oxidative stress, signaling, and pathophysiology, yet its highly dynamic and compartmentalized distribution in living cells makes precise monitoring a major analytical challenge.

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