Label-Free and Homogeneous Sensing Platform with Template-Independent Cascade Amplification Strategy for Genome-Wide 5-Hydroxymethylcytosine Analysis

Key Points

• This research aims to develop a sensing platform for accurate analysis of 5-hydroxymethylcytosine across the genome.
• Utilized a label-free sensing strategy.
• Employed a cascade amplification technique without template dependence.
• Evaluated the performance using receiver operating characteristic (ROC) curve analysis.
• Achieved an area under the ROC curve (AUC) of 0.993, indicating high sensitivity and specificity.
• Demonstrated potential applications in tumor liquid biopsies and epigenetic screening.