What does this research mean for the field?

Specific DNA extraction methods yield higher quality high-molecular-weight DNA necessary for successful PacBio HiFi long-read sequencing in the freshwater mussel Anodonta anatina. Novelty: ClaimNovelty.METHODOLOGICAL. Consensus alignment: ConsensusAlignment.NEUTRAL.

What question did this study set out to answer?

This response aims to address the effectiveness of various DNA extraction methods for PacBio HiFi sequencing in Anodonta anatina.

May 17, 2026Peer Community In Genomics0 citationsOpen Access

Author response of: Comparing DNA extraction methods for successful PacBio HiFi sequencing: a case study of the freshwater mussel Anodonta anatina (Bivalvia: Unionidae). Round#2

MGMarco Giulio ULUrs Lergster PFPhiline G. D. Feulner

Key Points

This response aims to address the effectiveness of various DNA extraction methods for PacBio HiFi sequencing in Anodonta anatina.
Comparison of different DNA extraction protocols.
Evaluation of their impact on sequencing quality.
Case study focused on freshwater mussel Anodonta anatina.
Specific extraction methods resulted in higher quality DNA for sequencing.
Enhanced read accuracy and completeness were noted with optimized protocols.
Findings support best practices for future genetic studies in mollusks.

Abstract

A recommendation of: Marco Giulio, Urs Lergster, Philine G. D. Feulner, Alexandra A. T. Weber Comparing DNA extraction methods for successful PacBio HiFi sequencing: a case study of the freshwater mussel Anodonta anatina (Bivalvia: Unionidae) https://doi.org/10.1101/2025.11.26.690746

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