The promoter of the guanylate-binding protein gene contains an IFN-alpha-stimulated response element and an overlapping IFN-gamma activation site required for maximal response to IFN-gamma.
This basic science study identifies specific overlapping promoter elements required for the transcriptional induction of the GBP gene by type I and type II interferons.
The gene encoding a 67-kDa cytoplasmic guanylate-binding protein (GBP) is transcriptionally induced in cells exposed to interferon of either type I (alpha interferon IFN-alpha or type II (IFN-gamma). The promoter of the GBP gene was cloned and found to contain an IFN-alpha-stimulated response element, which mediated the response of the GBP gene to IFN-alpha. On the basis of transfection experiments with recombinant plasmids, two different elements were delineated. Both were required to obtain the maximal response of the GBP gene to IFN-gamma: the IFN-alpha-stimulated response element and an overlapping element termed the IFN-gamma activation site. Different proteins that act on each element were investigated, and their possible involvement in IFN-gamma-induced transcriptional regulation is discussed.
Lew et al. (1991) studied this question. Interferon alpha and gamma was evaluated on Transcriptional induction of the GBP gene. The promoter of the guanylate-binding protein gene contains an IFN-alpha-stimulated response element and an overlapping IFN-gamma activation site required for maximal response to IFN-gamma.