AAV2 replication compartments recruit specific cellular proteins involved in DNA replication, repair, and RNA splicing, with the protein profile varying depending on the helper virus used.
This review discusses the cellular proteins involved in AAV2 replication compartments and their potential roles in AAV2 and helpervirus infection.
Adeno-associated virus 2 (AAV2) is a helpervirus-dependent parvovirus with a bi-phasic life cycle comprising latency in absence and lytic replication in presence of a helpervirus, such as adenovirus (Ad) or herpes simplex virus type 1 (HSV-1). Helpervirus-supported AAV2 replication takes place in replication compartments (RCs) in the cell nucleus where virus DNA replication and transcription occur. RCs consist of a defined set of helper virus-, AAV2-, and cellular proteins. Here we compare the profile of cellular proteins recruited into AAV2 RCs or identified in Rep78-associated complexes when either Ad or HSV-1 is the helpervirus, and we discuss the potential roles of some of these proteins in AAV2 and helpervirus infection.
Rebecca Vogel (Fri,) conducted a review in AAV2 replication. AAV2 replication compartments recruit specific cellular proteins involved in DNA replication, repair, and RNA splicing, with the protein profile varying depending on the helper virus used.