Abstract The study was conducted in the laboratories of the College of Life Sciences at the University of Kufa, with the aim of investigating the effects of red apple seed extract (Malus pumila Mill) on the liver and kidneys, as well as its potential to inhibit cancer cells. Additionally, the study sought to identify and detect secondary compounds present in the extract using white laboratory rats (male) weighing between 100 and 150 grams. A total of 20 rats were used in the study, divided into four groups, each consisting of five male rats. The first group received 0.5 grams of fresh apple seed extract. Observations and procedures were carried out over a period of seven days, and tissue samples were subsequently collected. The second group was administered a doubled dose of 1 gram for two weeks. Samples from this group were then collected. Based on the observed changes and to determine the dose at which tissue damage or fatal harm could occur, the third group was given 1 gram of the extract. Samples from this group were delivered to Al-Iman Laboratory in Najaf Governorate for further analysis. Various tests were performed, including Fourier-transform infrared spectroscopy (FTIR), enzyme-linked immunosorbent assay (ELISA), and other reagent-based assays, to analyze the effects and detect the presence of secondary compounds in the apple seed extract.
Manar Saeb Najeeb (Wed,) studied this question.