Cultured macrophages synthesize and constitutively secrete lipoprotein lipase, a process that is blocked by cycloheximide treatment.
Cultured macrophages synthesize and secrete lipoprotein lipase, suggesting a potential role for these cells in atherogenesis.
Cultured macrophages were shown to synthesize and constitutively secrete a triglyceride lipase identified as lipoprotein lipase on the basis of the following criteria: 1) it required apolipoprotein C-II for optimal activity; 2) it exhibited maximal activity at pH 8.2; 3) it was inhibited by 1 M NaCl; 4) it bound tightly to a heparin-Sepharose affinity gel and was eluted with buffer containing 1.5 M NaCl; and 5) the quantity of enzyme released from macrophages was enhanced by heparin. Cycloheximide treatment of macrophage cultures blocked the spontaneous secretion of lipoprotein lipase. Macrophage secretion of lipoprotein lipase may be relevant to the role of this cell in atherogenesis.
Khoo et al. (1981) studied this question. Cycloheximide treatment vs. Untreated macrophages was evaluated on Secretion of lipoprotein lipase. Cultured macrophages synthesize and constitutively secrete lipoprotein lipase, a process that is blocked by cycloheximide treatment.