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July 1, 1975Proceedings of the National Academy of Sciences168 citationsOpen Access

Modification of the 5'-terminus of mRNA by soluble guanylyl and methyl transferases from vaccinia virus.

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MEM J EnsingerSMScott A. MartinEPEnzo Paoletti

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Abstract

RNA guanylyl and methyl transferases have been solubilized from vaccinia virus cores. The guanylyl transferase specifically adds a GMP residue to the 5'-terminus of unmethylated vaccinia virus mRNA to form the structures G(5')ppp(5')Gp- and G(5')ppp(5')Ap-. Studies with alpha-32PGTP and beta, gamma-32PGTP indicated that only the alpha-phosphate is transferred. In the presence of S-adenosylmethionine, the methyl transferases convert the blocked 5'-termini to m7G(5')ppp(5')Gmp- and m7G(5')ppp(5')Amp-. Similarly, the enzymes can modify synthetic poly(A) to form the structure m7G(5')ppp(5')Amp-.

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Cite This Study

Ensinger et al. (1975) studied this question.

synapsesocial.com/papers/6a2083687b2df09761f8988fhttps://doi.org/10.1073/pnas.72.7.2525
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