Does treatment with Tween 80 and ether increase hemagglutinating activity in measles virus tissue culture material?
Treatment of measles virus tissue culture material with Tween 80 and ether significantly increases hemagglutinating activity, providing a simple method for producing high-potency antigen for HI tests.
A simple method for increasing hemagglutinating activity of measles virus tissue culture material is described. By treatment with Tween 80 and ether a 4- to 8-fold increase in titer was obtained with virus material grown in a human embryonic cell line (Lu 106) and an 8- to 32-fold increase when virus was grown in dog kidney TC. Maximal HA titers obtained with unconcentrated material were respectively 1:1024 and 1:4096 per 0.4 ml with the two different materials. Serum titers greater by a factor of 2 were obtained when Tween and ether treated material was substituted for untreated material as antigen in HI tests.
Erling Norrby (Sat,) studied this question.
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