Metformin通过下调Hedgehog途径缓解高血糖引起的内皮损伤

; BECN1 = beclin 1; CDH5/VE-cadherin = cadherin 5; CASP3 = caspase 3; CASP8 = caspase 8; CASP9 = caspase 9; ECs = 内皮细胞; GAPDH = 甘油醛-3-磷酸脱氢酶; GCL = 神经节细胞层; GFP-LC3B = 绿色荧光蛋白标记的LC3B; HG = 高葡萄糖; Hh = Hedgehog; HHIP = Hedgehog相互作用蛋白; HUVECs = 人脐静脉内皮细胞; IB4 = 异植物凝集素B4; INL = 内核层; i.p. = 腹腔内; MAP1LC3/LC3 = 微管相关蛋白1轻链3; MAN = 甘露醇; MET = 二甲双胍; NG = 正常葡萄糖; ONL = 外核层; p-ACAC = 磷酸化乙酰辅酶A羧化酶; PECAM1/CD31= 血小板/内皮细胞粘附分子1; PRKAA1/2 = 蛋白激酶AMP-活化催化亚基α 1/2; p-PRKAA1/2 = 磷酸化PRKAA1/2; PTCH1 = patched 1; RAPA = 雷帕霉素; RL = Renilla荧光素酶; SHH = sonic hedgehog; shRNA = 短发夹RNA; sh-PRKAA1/2 = 针对人PRKAA1/2的短发夹RNA; scrambled shRNA = 随机短发夹RNA作为针对特定短发夹RNA的阴性对照，与输入序列具有相同的核苷酸组成，并且与所选生物体数据库的任何mRNA无匹配; SMO = smoothened, frizzled类受体; sqRT-PCR = 半定量RT-PCR; TEK/Tie2 = TEK受体酪氨酸激酶; Tek-Cre (+)小鼠 = 表达Cre重组酶的鼠株，在TEK的启动子/增强子的控制下以全内皮的方式; TUNEL = 末端去氧核苷酸转移酶dUTP介导的缺口末端标记.