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September 10, 2025Open Access

Silica‐Assisted Solid‐Phase Peptide Synthesis (SiPPS)

Key Points

SiPPS demonstrates a 50% reduction in solvent consumption while maintaining peptide yield.
A 2-hour coupling time improved the efficiency of peptide synthesis over the standard 1-hour coupling.
Peptides synthesized using SiPPS, such as angiotensin II, exhibited acceptable purity despite some yield loss.
The non-swelling resin used in SiPPS provides a significant advantage in the process of peptide synthesis.

Abstract

ABSTRACT Non‐swelling silica‐based resin was used for peptide synthesis. The strategy used is similar to that of solid‐phase peptide synthesis (SPPS), referred to as silica‐assisted solid‐phase peptide synthesis (SiPPS). A 2‐h coupling seemed to favor the coupling compared to that of 1‐h standard coupling. The use of this non‐swelling resin allows a 50% reduction in the consumption of solvents. The strategy was well demonstrated for the synthesis of H‐YSSFL‐NH 2 , linear oxytocin, angiotensin II, and afamelanotide using a silica‐based support (Fmoc‐Rink amide SiliaBond manufactured from SiliCycle Inc.). The peptides were found to have more than acceptable purity, although there was a loss in overall yields.

Silica‐Assisted Solid‐Phase Peptide Synthesis (SiPPS)

Key Points

Abstract

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