Silica‐Assisted Solid‐Phase Peptide Synthesis (SiPPS)

Non‐swelling silica‐based resin was used for peptide synthesis. The strategy used is similar to that of solid‐phase peptide synthesis (SPPS), referred to as silica‐assisted solid‐phase peptide synthesis (SiPPS). A 2‐h coupling seemed to favor the coupling compared to that of 1‐h standard coupling. The use of this non‐swelling resin allows a 50% reduction in the consumption of solvents. The strategy was well demonstrated for the synthesis of H‐YSSFL‐NH 2 , linear oxytocin, angiotensin II, and afamelanotide using a silica‐based support (Fmoc‐Rink amide SiliaBond manufactured from SiliCycle Inc.). The peptides were found to have more than acceptable purity, although there was a loss in overall yields.