This research aims to enhance the efficiency of prime editing to confer herbicide resistance in peanuts.
Utilized prime editing technique to target acetolactate synthase gene.
Introduced W574L mutation to assess its impact on herbicide resistance.
Analyzed editing efficiency and mutation outcomes.
Confirmed that the W574L mutation provides broad-spectrum herbicide resistance.
Editing efficiency observed was suboptimal and highlighted for further improvement.
Abstract
Prime editing of acetolactate synthase to introduce the W574L mutation confers broad-spectrum herbicide resistance in peanut, though efficiency remains suboptimal and requires further refinement.