A complete cloned cDNA copy of the poliovirus RNA genome is infectious when transfected into mammalian cells, establishing a system for genetic manipulation of the virus.
A complete, cloned complementary DNA copy of the RNA genome of poliovirus was constructed in the Pst I site of the bacterial plasmid pBR322. Cultured mammalian cells transfected with this hybrid plasmid produced infectious poliovirus. Cells transfected with a plasmid which lacked the first 115 bases of the poliovirus genome did not produce virus.
Racaniello et al. (Fri,) studied this question.
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