November 20, 1981Science
Cloned Poliovirus Complementary DNA Is Infectious in Mammalian Cells
Population
Cultured mammalian cells
Comparison
Transfection with a complete, cloned… vs Transfection with a plasmid lacking the first…
Design
Preclinical
Authors
Discussion
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Implication
Enables reverse genetics for poliovirus in vitro; leaves open translation to animal models or clinical applications.
Structured PICO
P
Population
Cultured mammalian cells
I
Intervention
Transfection with a complete, cloned complementary DNA copy of the RNA genome of poliovirus constructed in the Pst I site of the bacterial plasmid pBR322
C
Comparator
Transfection with a plasmid lacking the first 115 bases of the poliovirus genome
O
Outcome
Production of infectious poliovirussurrogate
A complete cloned cDNA copy of the poliovirus RNA genome is infectious when transfected into mammalian cells, establishing a system for genetic manipulation of the virus.
Cite This Study
Racaniello et al. (1981) studied this question.